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Journal: Journal of Analytical Methods in Chemistry
Article Title: Capture-SELEX: Selection of DNA Aptamers for Aminoglycoside Antibiotics
doi: 10.1155/2012/415697
Figure Lengend Snippet: Affinity tests on the representatives of aptamer groups evolved from Capture-SELEX. Affinity tests were performed similar to the Capture-SELEX procedure, and ssDNA was eluted by different concentrations of kanamycin A in selection buffer. Data was fitted by the model of one site direct binding using a rectangular hyperbola for the saturation curve (OriginLab Corporation, OriginPro 8G SR2).
Article Snippet: These amounts are then quantified by fluorescence detection , and the resulting data is fitted by the model of one-site direct binding using a rectangular hyperbola also known as binding isotherm or saturation binding curve (
Techniques: Selection, Binding Assay